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| Gonçalves,Heloísa Bressan; Riul,Alana Jacomini; Quiapim,Andréa Carla; Jorge,João Atílio; Guimarães,Luis Henrique Souza. |
| Background: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH... |
| Tipo: Journal article |
Palavras-chave: Aspergillus ochraceus; Gallic acid; Tannase; Tannic acid; Tannin acyl hydrolase. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500004 |
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| Alegre,Ana Cláudia Paiva; Polizeli,Maria de Lourdes Teixeira de Moraes; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza. |
| The filamentous fungus Aspergillus caespitosus was a good producer of intracellular and extracellular invertases under submerged (SbmF) or solid-state fermentation (SSF), using agroindustrial residues, such as wheat bran, as carbon source. The production of extracellular enzyme under SSF at 30ºC, for 72h, was enhanced using SR salt solution (1:1, w/v) to humidify the substrate. The extracellular activity under SSF using wheat bran was around 5.5-fold higher than that obtained in SbmF (Khanna medium) with the same carbon source. However, the production of enzyme with wheat bran plus oat meal was 2.2-fold higher than wheat bran isolated. The enzymatic production was affected by supplementation with nitrogen and phosphate sources. The addition of glucose in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus caespitosus; ²-D-fructofuranosidase; Invertase; Solid-state fermentation; Submerged fermentation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300025 |
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| Silva,Tony Márcio; Oliveira,Maurício de; Somera,Alexandre Favarin; Jorge,João Atílio; Terenzi,Héctor Francico; Polizeli,Maria de Lourdes T. M; Guimarães,Luis Henrique Souza. |
| The effect of several nutritional and environmental parameters on Penicillium purpurogenum growth and sacharogenic amylase production was analyzed. High enzyme levels (68.2 U mg-1) were obtained with Khanna medium at initial pH 6.0, incubated at 30ºC for 144 hours. The optimum pH and temperature activities were 5.0 and 65ºC, respectively. The enzyme presented a half-life (t50) of 60 min, at 65ºC. Only glucose was detected after 24 hours of reaction using soluble starch as substrate. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amylase; Penicillium purpurogenum; Submerged fermentation. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300035 |
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